AAS ›› 2013, Vol. 44 ›› Issue (4 ): 514-518.doi: 10.3969/j.issn.0529-1356.2013.04.013

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Comparision of observations between coronal histological and CT images of the lateral skull base

LI Xi-ping1 XIA Yin2 HAN De-min 2*   

  1. 1.Department of Otolaryngology Head and Neck Surgery, Beijing Anzhen Hospital Affiliated of Capital Medical University,Beijing 100029, China; 2.Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital Affiliated of Capital Medical University,Beijing 100730, China
  • Received:2012-09-19 Revised:2012-11-18 Online:2013-08-06 Published:2013-09-04

Abstract:

Objective To obtain digital image data from sequential sectional coronary celloidin slices of the temporal bone in order to provide references for imaging sectional study and clinical diagnosis of the lateral skull base. Methods Two cadaveric
heads were used to make sectional specimens after spiral CT scanning. After celloidin embedding, a brain slicer was used for making thin slices(100 μm). Digital camera was used to take pictures after each slicing.Representative slices were stained by
HE method.Digital pictures,HE pictures and CT images were examined and comparied to identify characteristics of sectional structures. Results Two sets of sequential digital images were obtained with a resolution of 1920×2560 and spatial relationship among different
structures was clearly shown. Sectional structures were demonstrated more clearly after contrasting observation. Conclusion Celloidin embedding is an ideal method for obtaining high resolution sequential digital imaging data of the lateral skull base.
Coronary images are suitable to show ossicles, Prussak’s space,jugular vein fossa,the relationship between cochlear and middle cranial fossa, the jugular and petrous segment of internal carotid artery,the vertical segment of facial nerve,the relationship
between internal acoustic canal and middle cranial fossa.

Key words: Lateral skull base, Temporal bone, Celloidin, Anatomy, Section, Human